Tshiab Tech lub siab tsis siv neeg nucleic acid extraction thiab nrhiav kom tau tshuab

Tsib lub zog:
● Cov cuab yeej teeb tsa nrog cov nucleic acid extraction thiab purified kauj ruam
● Instrument configured nrog ultrasonic extraction module
● Cov cuab yeej teeb tsa nrog lub siab tsis siv neeg
● Cov cuab yeej teeb tsa nrog qhov ntsuas kub hloov pauv
● Cov cuab yeej teeb tsa nrog cov khoom siv reagent tag nrho

1.Do nucleic acid nrhiav kom tau reagents yuav tsum tau muab rho tawm thiab purification?
Lub hauv paus ntsiab lus ntawm kev tshawb nrhiav nucleic acid yog raws li hauv qab no: nyob rau hauv qhov kev txiav txim ntawm primer, DNA polymerase yog siv los ua cov tshuaj tiv thaiv kab mob ntawm cov qauv DNA / RNA (yuav tsum tau thim rov qab ntawm NA), thiab tom qab ntawd tus nqi ntawm cov teeb liab fluorescent tso tawm los txiav txim siab. seb tus qauv twg muaj cov nucleic acid (DNA/RNA) ntawm cov kab mob kom kuaj tau.

1) Cov qauv uas tsis tau muab rho tawm lossis ua kom huv tuaj yeem muaj ntau yam cuam tshuam rau qhov kawg tshwm sim: nuclease (uas tuaj yeem yaj lub hom phiaj nucleic acid thiab ua rau tsis zoo), protease (uas tuaj yeem txo DNA polymerase thiab ua rau tsis zoo), cov hlau hnyav ntsev (uas ua rau inactivation ntawm synthase thiab ua rau tsis muaj tseeb zoo), acidic los yog alkaline PH (uas yuav ua rau cov tshuaj tiv thaiv tsis tiav), RNA tsis tiav (ua rau tsis zoo tsis zoo rov qab transcription tsis ua hauj lwm).

2) Qee cov qauv tsis yooj yim rau kev nthuav dav ncaj qha: Gram-positive thiab qee cov kab mob cab, vim tias lawv cov phab ntsa tuab thiab lwm cov qauv, yog tias lawv tsis mus dhau ntawm nucleic acid extraction thiab purification txheej txheem, cov khoom siv rho tawm tsis pub dawb yuav ua tsis tiav rau qhov no. cov qauv.

Yog li ntawd, nws raug nquahu kom xaiv cov khoom siv kuaj lossis ntsuas ntsuas nrog nucleic acid extraction kauj ruam.

2. Tshuaj extraction los yog lub cev ultrasonic fragmentation extraction?
Feem ntau hais lus, tshuaj extraction tuaj yeem siv rau feem ntau ntawm pretreatment thiab Purification.Txawm li cas los xij, nyob rau hauv tuab-walled Gram-positive kab mob thiab qee cov kab mob parasites, nws tseem yog cov ntaub ntawv uas cov tshuaj rho tawm tsis tuaj yeem tau txais cov qauv nucleic acid zoo, uas ua rau pom qhov tsis zoo.Tsis tas li ntawd, kev tshem tawm tshuaj lom neeg feem ntau siv cov tshuaj muaj zog, yog tias qhov elution tsis meej, nws yog ib qho yooj yim los qhia muaj zog alkali rau hauv cov tshuaj tiv thaiv kab mob, ua rau cov txiaj ntsig tsis raug.

Ultrasonic fragmentation siv lub cev crushing, uas tau ntse siv los ntawm GeneXpert, ib tug ua lag luam nyob rau hauv lub teb ntawm POCT rau tib neeg siv, thiab muaj ib tug zoo kom zoo dua ntawm nucleic acid extraction ntawm ib co complex qauv (xws li Mycobacterium tuberculosis).

Yog li ntawd, nws raug nquahu kom xaiv cov khoom siv kuaj lossis ntsuas ntsuas nrog nucleic acid extraction kauj ruam.thiab nws yog qhov zoo tshaj yog tias muaj ultrasonic extraction module.

3. Phau ntawv, Semi-automatic thiab tag nrho tsis siv neeg?
Qhov no yog qhov teeb meem ntawm tus nqi zog thiab kev ua haujlwm ntawm kev ua haujlwm.Tam sim no, cov tsev kho mob tsiaj tsis muaj neeg ua haujlwm txaus, thiab kev tshem tawm nucleic acid thiab kuaj pom yog ib txoj haujlwm uas yuav tsum muaj qee yam kev txawj ntse thiab kev paub dhau los.Tsis muaj qhov tsis ntseeg tias lub tshuab tsis siv neeg nucleic acid extraction thiab nrhiav kom tau yog qhov kev xaiv zoo tshaj plaws.

4. Qhov ntsuas kub tsis tu ncua lossis qhov ntsuas kub hloov pauv tsis tu ncua?
Cov tshuaj tiv thaiv amplification yog qhov txuas nucleic acid nrhiav pom, thiab cov cuab yeej tshaj lij kev koom tes hauv qhov txuas no yog qhov nyuaj.Hais lus ntxhib, enzymes yog siv los nthuav cov nucleic acid.Nyob rau hauv cov txheej txheem amplification, lub amplified fluorescence teeb liab los yog embedded fluorescence teeb liab raug kuaj pom.Feem ntau hais lus, qhov ua ntej lub teeb liab fluorescence tshwm, qhov ntau dua lub hom phiaj cov ntsiab lus ntawm cov qauv.

Qhov ntsuas kub tsis tu ncua yog ib qho nucleic acid amplification ntawm qhov kub thiab txias, thaum qhov ntsuas kub hloov pauv hloov pauv yog ib qho kev ua kom nrawm nrawm raws li denaturation-annealing-extension.Lub sijhawm ntsuas kub tsis tu ncua tau ua tiav, thaum lub sijhawm hloov pauv kub hloov pauv tau cuam tshuam zoo heev los ntawm qhov ntsuas kub nce thiab poob ntawm qhov ntsuas (tam sim no, ntau lub tuam txhab tau ua 40 lub voj voog ntawm kev nthuav dav hauv li 30 feeb).

Yog tias qhov chaw kuaj mob zoo thiab qhov zoning nruj, nws tsim nyog hais tias qhov tseeb qhov sib txawv ntawm ob yuav tsis zoo.Txawm li cas los xij, qhov ntsuas kub hloov pauv hloov pauv yuav ua rau cov khoom lag luam nucleic acid ntau dua nyob rau lub sijhawm luv dua.Rau cov chaw soj nstuam uas tsis muaj kev txwv nruj thiab cov neeg ua haujlwm cob qhia, qhov kev pheej hmoo ntawm nucleic acid aerosol to yuav ntau dua, qhov tsis zoo tshwm sim thaum muaj qhov tshwm sim, thiab qhov ntawd yog qhov nyuaj heev kom tshem tawm.

Tsis tas li ntawd, qhov ntsuas kub tsis tu ncua kuj tseem muaj ntau dua rau cov tsis muaj kev cuam tshuam tshwj xeeb thaum tus qauv yog qhov nyuaj (qhov ntsuas kub ntawm cov txheeb ze qis dua, thiab qhov siab dua qhov txuas ntxiv, qhov zoo dua cov primer binding specificity).

Raws li cov cuab yeej siv tam sim no muaj kev txhawj xeeb, qhov ntsuas kub hloov pauv tau yooj yim dua.

5. Yuav ua li cas kom tsis txhob muaj kev pheej hmoo ntawm kev xau ntawm nucleic acid amplification khoom?
Tam sim no, ntau lub tuam txhab xaiv lub caj pas hom PCR raj raws li cov nucleic acid cov tshuaj tiv thaiv raj, uas yog kaw los ntawm kev sib txhuam, thiab qhov kub thiab txias denaturation nyob rau hauv qhov sib txawv kub denaturation nyob rau hauv qhov sib txawv kub PCR amplification ncav cuag 90 degree.
Centigrade.Cov txheej txheem rov ua dua ntawm kev nthuav dav nrog cov cua sov thiab kev cog lus nrog txias yog ib qho kev sib tw zoo rau kev sib khi ntawm PCR raj, thiab lub caj pas hom PCR raj yog ib qho yooj yim los ua kom tawg.

Nws yog qhov zoo dua los txais yuav cov tshuaj tiv thaiv nrog cov khoom siv kaw / raj kom tsis txhob xau ntawm cov khoom siv tshuaj tiv thaiv.Nws yuav zoo meej yog tias muaj peev xwm ua haujlwm tawm cov khoom ntim tag nrho rau nucleic acid extraction thiab kuaj pom.

Yog li New Tech tus tshiab tag nrho tsis siv neeg nucleic acid extraction thiab nrhiav kom tau tshuab muaj cov saum toj no tsib txoj kev xaiv.