Canine Cortisol (cCortisol) yog ib qho tshuaj steroid uas tsim los ntawm canine adrenal cortex.Cortisol pab tswj cov ntshav qab zib kom tsis tu ncua, txo qhov mob thiab pab lub cev tswj kev ntxhov siab.Txawm hais tias muaj ntau yam mob tshwm sim los ntawm qhov txawv txav ntawm cov tshuaj hormones cawv hu ua Cushing syndrome (CS), ob tus dev thiab miv tuaj yeem raug kev txom nyem los ntawm CS, uas muaj ntau dua hauv cov dev dua li miv.Cov dev ntawm hnub nyoog nruab nrab thiab laus (kwv yees li 7 txog 12 xyoos)
feem ntau yuav tsim tus kab mob.Tus kab mob no maj mam thiab cov tsos mob thaum ntxov tsis yog ib qho yooj yim mus kuaj.CS tuaj yeem kuaj pom los ntawm adrenocorticotropic hormone (ACTH) stimulation test thiab dexamethasone suppression test, thiab nws ntau hom: adrenal-dependent (ATH) thiab pituitary-dependent (PDH).
Cov khoom no siv cov tshuaj fluorescence immunochromatography kom pom cov ntsiab lus ntawm cCortisol hauv dev ntshav / ntshav.Lub hauv paus ntsiab lus: T thiab C kab yog cim rau ntawm nitrocellulose daim nyias nyias, T kab yog coated nrog cCortisol antigen a, thiab cov ntaub ntawv khi yog sprayed nrog ib tug fluorescent nanomaterial lo lo ntxhuav antibody b uas muaj peev xwm paub meej cCortisol.
nws cCortisol hauv cov qauv yog thawj zaug sau nrog nanomaterial.Cov tshuaj tiv thaiv b khi los ua ib qho nyuaj, thiab tom qab ntawd chromatographs upwards.Lub complex sib tw nrog T-line antigen a thiab tsis tuaj yeem ntes tau;Ntawm qhov tsis sib xws, thaum tsis muaj cCortisol hauv cov qauv, cov tshuaj tiv thaiv b khi rau antigen a.Thaum lub teeb excitation yog irradiated, cov khoom nano emits lub teeb liab fluorescent, thiab lub zog ntawm lub teeb liab yog inversely proportional rau cov concentration ntawm cCortisol nyob rau hauv cov qauv.
Txij li thaum nws tsim, peb lub Hoobkas tau tsim thawj cov khoom hauv ntiaj teb nrog kev ua raws li txoj cai
ntawm qhov zoo ua ntej.Peb cov khoom tau txais lub koob npe nrov hauv kev lag luam thiab muaj txiaj ntsig zoo ntawm cov neeg siv khoom tshiab thiab qub ..