Feline Health Marker Combined Detection (5-6 yam khoom)

【Tsim lub hom phiaj】
Feline pancreatic lipase (fPL): Lub txiav yog qhov thib ob loj tshaj plaws digestive caj pas nyob rau hauv cov tsiaj lub cev (thawj yog lub siab), nyob rau hauv lub plab mog pem hauv ntej ntawm lub cev, muab faib ua sab laug thiab sab xis lobes.Nws lub luag haujlwm tseem ceeb yog txhawm rau tso cov enzymes tsim nyog rau lub cev.Pancreatitis tau muab faib ua tus mob pancreatitis thiab mob pancreatitis.Qhov kev puas tsuaj los ntawm yav dhau los yog feem ntau ib ntus, thaum lub sij hawm tom kawg tawm mus tas li fibrosis thiab atrophy nyob rau hauv cov chav kawm ntawm rov qab mob.Ntawm lawv, tus kab mob pancreatitis ntev txog li 2/3 ntawm miv pancreatitis.
Cholyglycine (CG) yog ib qho ntawm cov cholic acids conjugated los ntawm kev sib xyaw ntawm cholic acid thiab glycine.Glycocholic acid yog qhov tseem ceeb tshaj plaws ntawm cov kua tsib acid hauv cov ntshav thaum cev xeeb tub.Thaum lub siab ua rau lub siab puas lawm, qhov nce ntawm CG los ntawm lub siab lub hlwb poob qis, ua rau muaj cov ntsiab lus CG hauv cov ntshav.Hauv cholestasis, kev tso tawm ntawm cholic acid los ntawm lub siab tsis zoo, thiab cov ntsiab lus ntawm CG rov qab mus rau cov ntshav ncig yog nce, uas tseem ua rau cov ntsiab lus ntawm CG hauv cov ntshav nce ntxiv.Bile acids khaws cia hauv lub gallbladder, uas tuaj yeem tshem tawm. los ntawm cov kab mob siab tom qab noj mov.Ib yam li ntawd, cov kab mob hauv siab thiab cov kab mob hauv lub cev tuaj yeem ua rau lub cev tsis zoo.
Cystatin C yog ib qho ntawm cov cystatin proteins.Lub luag haujlwm tseem ceeb tshaj plaws ntawm lub cev yog tswj hwm kev ua haujlwm ntawm cysteine ​​protease, uas muaj cov nyhuv inhibitory zoo tshaj plaws ntawm cathepsin B, papain, figs protease, thiab cathepsin H thiab kuv tso tawm los ntawm lysosomes.Nws ua lub luag haujlwm tseem ceeb hauv cov metabolism hauv intracellular peptides thiab cov proteins, tshwj xeeb tshaj yog nyob rau hauv cov metabolism hauv collagen, uas tuaj yeem hydrolyze qee cov prehormones thiab tso tawm rau hauv cov ntaub so ntswg los ua lawv txoj haujlwm lom neeg.Cov kab mob cerebral hemorrhage nrog amyloidosis yog ib yam kab mob ncaj qha ntsig txog cystatin C noob hloov, uas tuaj yeem ua rau cerebral vascular rupture, cerebral hemorrhage thiab lwm yam teeb meem loj.Lub raum tsuas yog qhov chaw kom tshem tawm cystatin C, thiab kev tsim cov cystatin C yog tas li.Cov ntshav cystatin C qib feem ntau yog nyob ntawm GFR, uas yog qhov zoo tshaj plaws endogenous marker los cuam tshuam cov kev hloov ntawm GFR.Cov kev hloov pauv hauv cov ntsiab lus ntawm lwm cov kua hauv lub cev kuj cuam tshuam nrog ntau yam kab mob.
NT-proBNP (N-terminal pro-brain natriuretic peptide), tseem hu ua B-hom diuretic peptide, yog cov tshuaj hormones protein secreted los ntawm cardiomyocytes hauv ventricles ntawm lub plawv.Thaum ventricular ntshav siab nce, ventricular dilation, myocardial hypertrophy, los yog siab ntawm myocardium nce, tus precursor ntawm NT-proBNP, proBNP (muaj 108 amino acids), yog secreted rau hauv cov hlab ntsha los ntawm cardiomyocytes.
Cat allergen tag nrho IgE (fTIgE): IgE yog ib yam ntawm immunoglobulin (Ig) nrog ib qho hnyav molecular ntawm 188kD thiab cov ntsiab lus tsawg heev hauv cov ntshav.Nws feem ntau yog siv rau kev kuaj mob ntawm kev tsis haum tshuaj.Tsis tas li ntawd, nws tseem tuaj yeem pab kho tus kab mob parasitic thiab ntau yam myeloma.1. Kev tsis haum tshuaj: thaum muaj kev tsis haum tshuaj, nws ua rau muaj kev ua xua lgE.Qhov siab dua qhov allergen lgE, qhov hnyav dua qhov kev tsis haum tshuaj.2. Kab mob Parasite: tom qab tus tsiaj tau kis los ntawm cov kab mob parasites, qhov ua xua lgE kuj tseem tuaj yeem nce ntxiv, uas feem ntau cuam tshuam rau kev ua xua me me los ntawm cov kab mob parasite proteins.Tsis tas li ntawd, qhov tshaj tawm tias muaj mob qog noj ntshav kuj tuaj yeem ua rau nce siab ntawm tag nrho IgE.
【Nrhiav txoj cai】
Cov khoom no siv fluorescence immunochromatography kom paub ntau npaum li cas cov ntsiab lus ntawm fPL / CG / fCysC / fNT-proBNP / fTIgE hauv cov ntshav miv.Lub hauv paus ntsiab lus tseem ceeb yog tias nitrocellulose daim nyias nyias yog cim nrog T thiab C kab, thiab T kab yog coated nrog antibody a uas tshwj xeeb paub txog cov antigen.Cov ntaub qhwv lub hnab yog txau nrog lwm cov tshuaj fluorescent nanomaterial uas sau cov tshuaj tiv thaiv kab mob b uas tuaj yeem paub txog cov antigen.Cov tshuaj tiv thaiv kab mob hauv cov qauv khi rau nanomaterial sau npe antibody b los ua ib qho nyuaj, uas tom qab ntawd khi rau T-line antibody A los tsim cov qauv sandwich.Thaum lub teeb excitation yog irradiated, nanomaterial emits fluorescent signals.Qhov kev siv ntawm lub teeb liab tau zoo correlated nrog antigen concentration hauv cov qauv.