Canine Health Markers Combined Detection (5-6 yam khoom)

【Tsim lub hom phiaj】
Canine pancreatic lipase (cPL): Canine pancreatitis yog ib qho kab mob inflammatory infiltrative ntawm tus txiav.Feem ntau, nws tuaj yeem muab faib ua tus mob pancreatitis thiab mob pancreatitis.Pancreatic neutrophil infiltration, pancreatic necrosis, peripancreatic rog necrosis, edema thiab raug mob tuaj yeem pom hauv mob pancreatitis.Pancreatic fibrosis thiab atrophy tuaj yeem pom nyob rau hauv mob pancreatitis.Piv nrog rau mob pancreatitis, mob pancreatitis ntev tsis muaj teeb meem, tab sis ntau zaus.Thaum cov dev raug kev txom nyem los ntawm tus mob pancreatitis, tus txiav txiav raug puas tsuaj, thiab qib ntawm pancreatic lipase hauv cov ntshav nce siab.Tam sim no, pancreatic lipase yog ib qho ntawm cov cim qhia zoo tshaj plaws ntawm qhov tshwj xeeb rau kev kuaj mob pancreatitis hauv dev.
Cholyglycine (CG) yog ib qho ntawm cov cholic acids conjugated los ntawm kev sib xyaw ntawm cholic acid thiab glycine.Glycocholic acid yog qhov tseem ceeb tshaj plaws ntawm cov kua tsib acid hauv cov ntshav thaum cev xeeb tub.Thaum lub siab ua rau lub siab puas lawm, qhov nce ntawm CG los ntawm lub siab lub hlwb poob qis, ua rau muaj cov ntsiab lus CG hauv cov ntshav.Hauv cholestasis, kev tso tawm ntawm cholic acid los ntawm lub siab yog qhov tsis zoo, thiab cov ntsiab lus ntawm CG rov qab mus rau cov ntshav ncig yog nce, uas kuj nce cov ntsiab lus ntawm CG hauv cov ntshav.
Cystatin C yog ib qho ntawm cov cystatin proteins.Txog tam sim no, Cys C yog cov tshuaj endogenous uas ua tau raws li qhov yuav tsum tau muaj ntawm tus cim zoo tshaj plaws endogenous GFR.Nws yog ib qho rhiab heev thiab tshwj xeeb Performance index rau kev ntsuam xyuas ntawm canine raum ua haujlwm.
N-terminal pro-brain natriuretic peptide (Canine NT-proBNP) yog ib yam khoom siv zais cia los ntawm cardiomyocytes hauv Canine ventricle thiab tuaj yeem siv los ua qhov ntsuas ntsuas rau lub plawv tsis ua haujlwm.Qhov concentration ntawm cNT-proBNP hauv cov ntshav sib cuam tshuam nrog qhov hnyav ntawm tus kab mob.Yog li ntawd, NT-proBNP tuaj yeem tsis tsuas yog ntsuas qhov mob hnyav thiab mob plawv tsis ua haujlwm, tab sis kuj tseem siv tau los ua qhov qhia txog nws qhov kev mob tshwm sim.
Canine allergen tag nrho IgE (cTIgE): IgE yog ib hom immunoglobulin (Ig) uas muaj qhov hnyav ntawm 188kD thiab cov ntsiab lus tsawg heev hauv cov ntshav.Nws feem ntau yog siv rau kev kuaj mob ntawm kev tsis haum tshuaj.Tsis tas li ntawd, nws tseem tuaj yeem pab kho tus kab mob parasitic thiab ntau yam myeloma.1. Kev tsis haum tshuaj: thaum muaj kev tsis haum tshuaj, nws ua rau muaj kev ua xua lgE.Qhov siab dua qhov allergen lgE, qhov hnyav dua qhov kev tsis haum tshuaj.2. Kab mob Parasite: tom qab tus tsiaj tau kis los ntawm cov kab mob parasites, qhov ua xua lgE kuj tseem tuaj yeem nce ntxiv, uas feem ntau cuam tshuam rau kev ua xua me me los ntawm cov kab mob parasite proteins.Tsis tas li ntawd, qhov tshaj tawm tias muaj mob qog noj ntshav kuj tuaj yeem ua rau nce siab ntawm tag nrho IgE.

【Nrhiav txoj cai】
Cov khoom no siv fluorescence immunochromatography kom paub ntau npaum li cas ntawm cPL / CG / cCysC / cNT-proBNP / cTIgE cov ntsiab lus hauv cov ntshav canine.Lub hauv paus ntsiab lus tseem ceeb yog tias nitrocellulose daim nyias nyias yog cim nrog T thiab C kab, thiab T kab yog coated nrog antibody a uas tshwj xeeb paub txog cov antigen.Cov ntaub qhwv lub hnab yog txau nrog lwm cov tshuaj fluorescent nanomaterial uas sau cov tshuaj tiv thaiv kab mob b uas tuaj yeem paub txog cov antigen.Cov tshuaj tiv thaiv kab mob hauv cov qauv khi rau nanomaterial sau npe antibody b los ua ib qho nyuaj, uas tom qab ntawd khi rau T-line antibody A los tsim cov qauv sandwich.Thaum lub teeb excitation yog irradiated, nanomaterial emits fluorescent signals.Qhov kev siv ntawm lub teeb liab tau zoo correlated nrog antigen concentration hauv cov qauv.